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a. Order mutants     b .Expression Pattern Lines Help
04Z11EC52
General information
This line was generated from rice cultivar Zhonghua 11.
Reporter gene: GFP.
Seed status: available.
Morphology
Semi-dwarf has been screened at tillering stage for T1 plant . Segregation (mutant: normal) ratio is 5:35 . Phenotype co-segregated with PCR marker (by You Changjun ).
Abiotic stress
All the T1 plants were normal to drought treatment as compared with control (by Zhu Xiaoyi).
Positive Assay
Positive Positive assay result of T0 plants: Positive. (by You Changjun)
Flanking Sequence
 
  • Entry_ prefix Types
  •  

    ATL

    ATosL

    ATosR

    ITL

    ITR

     

    TTL

    TTR

    TTosL

    TTosR

    HTL

  • Entry_postfix Types (different primer id)
  •  

    AC1

    ADS-2

    LBT2

    TRB2

    ULB3

     

    XLB3

    XRB3

    URB4

    PFRB4

    NTLB5

     

    TosRS

    SP6

    T7

     

     

  • Additional remarks for Entry_prefix
  •   The first letter means methods for flanking sequence
    ⊕A: Adaptor-PCR ⊕T: TAIL-PCR ⊕I: Inverse-PCR ⊕H: Hi TAIL-PCR
    The middle letters means TAGs
    ⊕T: T-DNA ⊕Tos: Tos17
    The last letter means Left or Right
  • Mutant phenotype(s) mentioned in this entry may not be necessarily linked to the gene(s) identified by the following flanking       sequence(s) due to possible multiple insertions.
  • Blue color is used for rice genomic sequence and orange for vector sequence. Black color is used for neither vector nor rice       genomic sequence that could be caused by recombination.
  •  
    TTL _04Z11EC52 _NTLB5
    Sequence1|Possible location: Chromosome 4 (by You Changjun)
    AAAACGTGCCGCAATGTGTTATTAGAGTTGCCTAAGCGTCAATGGTTTACACCACAGTGATCCCAAGAGTTGAGAAGCTA
    TCCTCCACACTCTTGACCGGACCATTGTAAACAATTAGACCTCCTTTTGCCAGAAGTATCAGATCATCAAACATGTTGTA
    CAATGTGTAGCTGAATAACACAAACAAGAAAATATTAGAAATCTTTCAGAAAGATGAGTACTATCTTCAGAAAAAGATGG
    TCTGAGAGTAACCTCGGTTGATGAACAACTGCGCAAACATTTACGCCTTCGAGCGCCTCATGACGTAGTGCTCGGAGGAG
    CAGTGGTGAAGAGGAGCTGTCCAGGCCAGAGGTTGGTTCATCCAGGATCAGAAGTGATGGCTCCATCACCATTTCAATAC
    CCACATTGACACGCTTCCTCTGTCCACCAGAGATCCCGCGCTTCTCCACAGTTCCAACCAATGAACTTCTGACTCCCTGA
    AGGTCCAGGGAGTCTATGACCCTCTCCACAGTGAGGACCCTTGTGGCGGTGTGCCGTTGTCGCAGAGAGCCTGAAGATAG
    TAGAAATGATAAGAATATGCTGAGCTAATTACTGAAATGGCAATCTGATATAAAATACTTCCTCCGTTTCAATTGTTTTG
    TTTTTGTTAAGGACAATGGATTGACCAGCATACTTTATTATGAATATGACTTATTGTGATACAAACGGCATAATCGTAAG
    TTAAGTACTTTTGGAACCAAAATTCTAACAAATTCAAATTTTTGGGTTACAAAATTCATATCCAGTTTAAGTTAATTGTT
    GGTCAAAAGATTGCCTTACCGAAACAAAATTGTCTTAATTTTGGAAACCAGGATATCGTATGGCAAGAAACCTTTTTTAT
    ATTAAAAAGTAACCTGCACTGACTGAACAAGGTCTTGCAGTGAATGCATGACCACATTCTTGGGAAACATCCAAGTATCT
    TTTGGAAACGC
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